The Biomembrane Lab / Mayer Group
Research in the Biomembrane Lab focuses on transport and signaling processes across biological membranes. The goal of the group is to increase the molecular understanding of these processes and ultimately to employ the ensuing insight to diagnose and possibly treat human disease. We are particularly interested in ion channel proteins that are involved in Alzheimer’s disease and in autoimmune diseases such as multiple sclerosis, rheumatoid arthritis, and type I diabetes mellitus, as well as in transporter proteins that are responsible for resistance to chemotherapy in treatment of cancer.
Current research focuses on five main projects:
- High-throughput profiling of ion channel activity in human lymphocytes as a biomarker for T cell-mediated autoimmune diseases
- Chip-based transport assays of single multidrug resistance transporters
- Screening for small molecules that inhibit neurotoxic ion channel activity by amyloid-beta (AB) peptides as a possible strategy to treat Alzheimer’s disease (AD)
- Nanopore-based sensing of biomolecular assemblies and artificial ion channels
- Interactions of proteins and therapeutic drugs with biomembranes and lipid rafts
Research in the Biomembrane Lab is carried out in a multidisciplinary approach; we combine expertise in biochemistry, cell biology, and immunology with state-of-the art approaches in biophysics, electrophysiology, optical single molecule detection, and surface chemistry. The lab works in teams; current members include two postdoctoral researchers, four graduate students, and three undergraduate students.
For more information, please visit: http://biomembrane.engin.umich.edu/index.html
21. Mayer M., Kriebel J. K., Tosteson M. T., Whitesides G. M. “Microfabricated Teflon membranes for low-noise recordings of ion channels in planar lipid bilayers”, Biophys. J., 2003, 85, 2684-2695
22. Yang J., Mayer M., Kriebel J. K., Garstecki P., Whitesides. G. M. “Self-assembled aggregates of IgGs as templates for the growth of clusters of gold nanoparticles”, Angew. Chem. Int. Ed., 2004, 43, 1555-1558
23. Mayer M., Yang J., Gracias D., Whitesides G. M. “Micropatterned agarose gels for stamping arrays of proteins and gradients of proteins”, Proteomics, 2004, 4, 2366-2376
24. Stevens M. M., Mayer M. (S.M.M. and M.M. contributed equally), Anderson D. G., Weibel D. B., Whitesides G. W., Langer R. “Direct patterning of mammalian cells onto porous tissue engineering substrates using agarose stamps”, Biomaterials, 2005, 26, 7636-7641
25. DiLuzio W. R., Turner L., Mayer M., Garstecki P., Weibel D. B., Berg H. C., Whitesides G. M. „Escherichia coli „drive on the right“, Nature, 2005, 435, 1271-1274
26. Weibel D. B., Garstecki P., Ryan D., DiLuzio W. R., Mayer M., Seto J. E., Whitesides G. M. “Microoxen: microorganisms to move microscale loads“, Proc. Nat. Acad. Sci. USA, 2005, 102, 11963-11967
27. Estes D. J., Mayer M. “Giant liposomes in physiological buffer using electroformation in a flow-through setup”, Biochim. Biophys. Acta, 2005, 1712, 152-160
28. Majd S., Mayer M. “Hydrogel-stamping of arrays of supported lipid bilayers with varying lipid compositions for screening of drug-membrane and protein-membrane interactions”, Angew. Chem. Int. Ed., 2005, 44, 6697-6700
29. Blake S., Mayer T., Mayer M., Yang J. “Monitoring chemical reactions using ion channel-forming peptides, ChemBioChem, 2006, 7, 433-435.
30. Uram J. D., Ke K., Hunt A. J., Mayer M. “Label-free affinity assays by rapid detection of immune complexes using nanopores”, Angew. Chem. Int. Ed., 2006, 45, 2281-2285
31. Estes D. J., Lopez S. R., Fuller A. O., Mayer M. “Triggering and observing the aggregation and fusion of lipid membranes in a microfluidic chamber”, Biophys. J., 2006, 91, 233-243.
32. Uram J. D., Ke K., Hunt A. J., Mayer M. “Submicrometer pore-based characterization and quantification of antibody-virus interactions”, Small, 2006, 2, 967-972.
33 Uram J. D., Mayer M. “Estimation of solid phase affinity constants using resistive pulses from nanoparticles”, Biosens. and Bioelectr., 2007, 22, 1556-1560
34 Capone R., Blake S., Rincon Restrepo M., Yang J., Mayer M. “Designing nanosensors based on charged derivatives of gramicidin A”, J. Am. Chem. Soc., 2007, 129, 9737-9745.
35 Mayer M., Semetey V., Gitlin I., Yang J., Whitesides G.M. "Quantifying protein-ligand interactions using ion channel-forming peptides" J. Am. Chem. Soc., 2008, 130, 1453-1465
36 Estes D.J., Memarsadeghi S., Lundy S.K., Mikol D.D., Fox D.A., Mayer M. "High-throughput profiling of ion channel activity in primary human lymphocytes" Anal. Chem., 2008, 80, 3728-3735.
37 Uram J. D., Ke K., Mayer M. "Noise and bandwidth of current recordings from submicrometer pores and nanopores" ACS Nano, 2008, 2, 857-872.
38 Majd S., Mayer M. "Generating arrays with high content and minimal consumption of functional membrane proteins" J. Am. Chem. Soc., 2008, 130, 16060–16064
39 Capone R., Prangkio P., Sauer A.M., Bautista M.R., Turner R.S., Yang J., Mayer M. "Alzheimer's Disease-Related Amyloid-Beta Ion Channels: Resolving a Controversy" Neurotoxicity Research, 2009, 16, 1–13
40 Horger K.S., Estes D.J., Capone R., Mayer M. "Films of agarose enable rapid formation of giant liposomes in solution of physiologic ionic strength" J. Am. Chem. Soc., 2009, 131, 1810–1819