Note that MNIMBS does not have a graduate program. However, our MNIMBS Faculty members co-mentor multidisciplinary students and we do offer a certificate program in nanobiology for Ph.D. students who pursue studies in a traditional home department such as Chemistry, Physics, Immunology, Biomedical Engineering, Materials Science etc.
So you would need to make your Ph.D. program application via normal channels to a home Department of your choice. You can then express your interest in pursuing a certificate in nanobiology to your home department.
Information on PhD programs and cost can be found at: http://www.umich.edu/
Details and how to go about doing the certificate. See our web site: http://www.nano.med.umich.edu/About/Rackham-Certificate.html Home page: http://www.nano.med.umich.edu/index.html
You can download metrics on current and former students / Fellows here (2011 data) to get an idea of the variety of specializations and collaborations involved with MNIMBS.
On Monday, April 18, 2011, we held our Herbert D. Doan Nanotechnology Symposium. The Symposium was followed by a student Poster session. The following prizes were awarded:
Huai-Ning Chang -FIRST PRIZE Graduate student
Department of Mechanical Engineering; Division of Cardiovascular Medicine & MNIMBS
Microfluidic immunoblotting for profiling inflammatory pathways
Jack Wang - SECOND PRIZE Graduate student
Department of Biomedical Engineering
Development of a Physiologically Relevant In Vitro Model of the Blood-Brain Barrier
Yen-Ling Lin -THIRD PRIZE GRAD
Dept. of Biomedical Engineering
Development of “Smart” Particles for Effective Gene Silencing in Head & Neck Cancer
Yasemin Durmaz - HONORABLE MENTION Graduate student
Department of Biomedical Engineering, Cellular Engineering & Nano-Therapeutics Laboratory
Rational Design & Synthesis of “Intelligent” Star-Shaped Polymers for Enhancing Intracellular Delivery of Nucleic Acids
Di Gao - HONORABLE MENTION Graduate student
Dept. of Chemistry
Electron Induced Dissociation (EID) of Singly Protonated Glycans
Current Graduate Students
Huai-Ning Chang, MSE
Masters in Mechanical Engineering 2011
PhD candidate, Biomedical Engineering
Project: Development and application of microfluidic techniques in clinical diagnostics
Advisor: Sascha N Goonewardena, MD
Research Fellow and Clinical Lecturer in Cardiology, Medical School
Huai-Ning Chang received her masters degree in Mechanical Engineering at the University of Michigan in 2011. Her current research focuses on application of microfluidic principles to molecular biology by developing a microfluidic protein immunoblotting nanodevice. This nanodevice has allowed us to probe inflammatory protein networks with increased sensitivity and higher throughput.
On April 18, 2011, Huai-Ning won First Prize Graduate student for her poster on her project, Microfluidic immunoblotting for profiling inflammatory pathways, at the Herbert D. Doan Nanotechnology Symposium.
M.S. Analytical Chemistry
Department of Chemistry (2008-present)
Project: Developing Nitric Oxide Release and Generation Materials for Antithrombotic and Antimicrobial Applications
Advisor: Mark E. Meyerhoff, PhD
Wenyi received her B.S. and M.S. in chemistry from Nanjing University. At Nanjing University, she’s interested in developing nanomaterials for biosensing and bioassays. Most of her research is related with developing porous materials for enzyme electrode and she also spent one year in University of Queensland (Brisbane, Australia) working on using porous silica nanoparticles for immunoassays. She’s now starting her fourth year graduate student in Professor Meyerhoff’s group and her research is aimed at preventing thrombus formation and bacterial infection for biomedical devices using nitric oxide release and generation materials.
Cai, W.; Wu, J.; Xi, C.; Ashe, A.J.; Meyerhoff, M.E.; Carboxyl-Ebselen-Based Layer-by-Layer Films as Potential Antithrombotic and Antimicrobial Coatings. Biomaterials., in press, doi:10.1016/j.biomaterials.2011.06.075
Cai, W. Y.; Feng, L. D.; Liu, S. H.; Zhu, J. J., Hemoglobin-CdTe-CaCO3@Polyelectrolytes 3D Architecture: Fabrication, Characterization, and Application in Biosensing. Adv. Funct. Mater. 2008, 18, (20), 3127-3136.
Cai, W. Y.; Gentle, I. R.; Lu, G. Q.; Zhu, J. J.; Yu, A. M., Mesoporous Silica Templated Biolabels with Releasable Fluorophores for Immurroassays. Anal. Chem. 2008, 80, (14), 5401-5406.
Cai, W. Y.; Xu, Q.; Zhao, X. N.; Zhu, J. H.; Chen, H. Y., Porous Gold-nanoparticle-CaCO3 Hybrid Material: Preparation, Characterization, and Application for Horseradish Peroxidase Assembly and Direct Electrochemistry. Chem. Mater. 2006, 18, (2), 279-284.
Wang, X. Y.; Cai, W. Y.; Jiang, L. P.; Zhu, J. J., The Construction of Ag NPs-Titanium Phosphate Hybrid Ultrathin Film and its Electrochemical Biosensing. Chin. J. Inorg. Chem. 2008, 24, (12), 1923-1927.
Xu, Q.; Cai, W. Y.; Zhu, J. J., Self-assembly of Horseradish Peroxidase on Biocompatible Gold Nanoparticles-vaterite Core-shell Composite and Its Direct Electrochemistry. Chem. Lett. 2005, 34, (6), 832-833.
Third Prize of Poster Competition, the 15th annual GLCACS conference, Chicago, May 14th, 2011
Cai W.; Meyerhoff, M.E., “Carboxyl-Ebselen-Based Layer-by-Layer Film: A Potential Antithrombotic and Antimicrobial Coating”, BioInterface 2011 Annual Symposium, Bloomington, Minnesota, October 24-26, 2011
Cai W.; Meyerhoff, M.E., “Carboxyl-Ebselen-Based Layer-by-Layer Coating for Nitric Oxide Generation”, society for biomaterials 2011 annual meeting & Exposition, Orlando, Florida, April 13-16, 2011
Former Graduate Students
Mallory van Dongen
B.S. Niagara University, 2009
PhD Candidate, Chemistry
Advisor: Mark Banaszak Holl, PhD
Mallory received a Bachelor's of Science Degree in Chemistry from Niagara University in 2009. At Niagara, she was involved in computational and organic chemistry research projects. She entered Michigan's chemistry graduate program in the fall of 2009 as a Rackham Merit Fellow. Mallory joined the Banaszak Holl group in 2010. Her work focuses on synthesizing and characterizing dendrimer drug delivery systems.
Silpe JE, Sumit M, Thomas TP, Huang B, Kotlyar A, van Dongen MA, Banaszak Holl MM, Orr BG, Choi SK. Avidity modulation of folate-targeted multivalent dendrimers for evaluating biophysical models of cancer targeting nanoparticles. ACS Chem Biol. 2013 Sep 20;8(9):2063-71. PMID: 23855478
Isolation and Characterization of Dendrimers with Precise Numbers of Functional Groups
D. G Mullen, E. L. Borgmeier, A. Desai, M. A. Van Dongen, M. Barash, X.-M. Chen, J. R. Baker, B. G. Orr, M. M. Banaszak Holl Chemistry a European Journal 2010, 10, 10675-10678.
Ronny Priefer, Kathryn E. Leach, Todd D. Krauss, Jeanette R. Drapo, Michelle L. Ingalsbe, Mallory A. van Dongen, John C. Cadwalader, Megan A. Baumler, Matthew S. Pinto "Multilayer Film Preparation of PVPh from Aqueous Media" Surface and Coatings Technology, 2008, 202, 6109-6112.
Vincent M. Carroll, Megan A. Baumler, John C. Cadwalader, Jeanette R. Drapo, Michelle L. Ingalsbe, Matthew S. Pinto, Mallory A. van Dongen, Ronny Priefer "Synthesis of Poly(4-vinylphenol) Derivatives and Preparation of Multilayered Films from Aqueous Media" Polymeric Materials: Science and Engineering, 2007, 96, 488-489.
Angela Liu, B.S.
Masters in Public Health, Hospital and Molecular Epidemiology (-present)
Project: Nanoemulsion Based Vaccine for Chronic Hepatitis B Virus - Course work in our lab: EPID 565
Advisor: Douglas Smith, PhD
Research Associate Professor, Internal Medicine & MNIMBS
Angela graduated in 2010 from Indiana University in Bloomington with a B.S. in Biology, a B.A. in French, and a minor in Chemistry. She is currently a Masters in Public Health graduate student here at the University of Michigan studying Hospital and Molecular Epidemiology. Angela is currently working on the STTR Hepatitis B Nano-Emulsion project with Dr. Doug Smith.
University of Science and Technology of China
Department of Chemistry (2008-present)
Project: Collagen fibril morphology in healthy and estrogen depleted ovine bone
Advisor: Mark Banaszak Holl, PhD
About Flora: Flora received a B.S. in chemistry from University of Science and Technology of China in 2008. At USTC, she did research using state of the art Guassian 3 to study quantitative structure-property relationship of tetrathiafulvalene analogs. Here at Michigan, Flora is a materials chemistry graduate student advised by professor Mark Banaszak Holl. Her research is aimed at understanding bone collagen changes occurring in sheep and humans with osteoporosis disease.
Honors and Awards
2006-2007 Outstanding Student Scholarship(Grade 3)
2005-2006 Outstanding Student Scholarship(Grade 1)
2004 Outstanding Freshman Scholarship(Grade 2)
A Quantitative Assessment of Nanoparticle-Ligand Distributions: Implications for Targeted Drug and Imaging Delivery in Dendrimer Conjugates, D. G. Mullen, M. Fang, A. M. Desai, J. R. Baker, B. G. Orr, and M. M Banaszak Holl ACS Nano 2010, 4, 657-670.
Effect of Mass Transport in the Synthesis of Partially Acetylated Dendrimer: Implications for Functional Ligand-Nanoparticle Interactions, D. G Mullen, E. L. Borgmeier, M. Fang, D. Q. McNerny, A. Desai, J. R. Baker, B. G. Orr, M. M. Banaszak Holl Macromolecules 2010, 43, 6577-6587.
Type 1 Collagen Exists as a Distribution of Nanoscale Morphologies in Teeth, Bones, and Tendons, J. M. Wallace, Q. Chen, M. Fang, B. Erickson, B. G. Orr, M. M. Banaszak Holl Langmuir 2010, 26, 7349-7354.
Mullen, D.; Fang, M.; Desai, A.; Baker, J.; Orr, B. G.; Banaszak Holl, M. M., A Quantitative Assessment of Nanoparticle Ligand Distributions: Implications for Targeted Drug and Imaging Delivery in Dendrimer Conjugates. ACS Nano, in review 2009.
Wallace, J. M.; Chen, Q.; Fang, M.; Erickson, B.; Orr, B. G.; Banaszak Holl, M. M.,
Lack of Tissue-Specific Differences in the Distribution of Type I Collagen Fibril Morphologies. Langmuir, Submitted 2009.
Fang, M.; Li, Z.; Fu, Y., Substituent Effect on C-NO2 and N-NO2 Bone Dissociation Energies of Nitroaromatic Compounds. Chinese Journal of Chemistry, 2008, 26, (6), 1122-1128.
Fang, M; Wallace, J. M.; Orr, B. G.; Banaszak Holl, M. M., AFM Imaging and Quantitative Analysis of Ovine Type I Collagen Fibril. MNIMBS symposium, 2009.
B.S. Engineering Physics
Colorado School of Mines
M.S. Applied Physics
Colorado School of Mines
Project: Detection of DNA through the Asynchronous Rotation Tecnique
Advisor: Raoul Kopelman, PhD
About Alex: Alex graduated from Colorado School of Mines in 2007 with a B.S. in Engineering Physics and in 2008 with a M.S. in Applied Physics. He entered the University of Michigan Biophysics Graduate Program in 2008. His thesis project involves detection of DNA through the use of rotating magnetic particles.
Adams DE, Planchon TA, Hrin A, Squier JA, Durfee CG. Characterization of coupled nonlinear spatiospectral phase following an ultrafast self-focusing interaction. Optics Letters, 2009.
Minors in Mathematics and Computer Science
University of Turku, Finland
Applied Physics (2007-present)
Project: Emerging Microbiological Sensor - Asynchronous Rotation of Magnetic Beads
Advisor: Raoul Kopelman, PhD
About Paivo: Paivo received his BS from the University of Turku after completing a year long exchange program at the University of Michigan in 2007. He started Applied Physics program at the UofM the same year, joining Professor Kopelman's research group in 2008 where he currently is a doctoral candidate.
Kinnunen, P., I. Sinn, B. H. McNaughton, and R. Kopelman. 2010. High frequency asynchronous magnetic bead rotation for improved biosensors. Applied Physics Letters 97, no. 22. doi:10.1063/1.3505492.
Kinnunen, P., I. Sinn, B. H. McNaughton, D. W. Newton, M. A. Burns, and R. Kopelman. 2011. Monitoring the growth of individual bacteria using asynchronous magnetic bead rotation sensors. Biosensors and Bioelectronics. doi:10.1016/j.bios.2010.10.010.
Kelly, C.V., M. T. Kober, P. Kinnunen, D. A. Reis, B. G. Orr, and M. M. Banaszak Holl. 2009. Pulsed-laser creation and characterization of giant plasma membrane vesicles from cells. Journal of Biological Physics 35, no. 3 (6): 279-295. doi:10.1007/s10867-009-9167-7.
Hecht, A., P. Kinnunen, B. H McNaughton, and R. Kopelman. 2011. Label-acquired magnetorotation for biosensing: An asynchronous rotation assay. Journal of Magnetism and Magnetic Materials 323, no. 3 (February): 272-278. doi:10.1016/j.jmmm.2010.09.014.
McNaughton, Brandon H., Miri Shlomi, Paivo Kinnunen, Codrin Cionca, Shao Ning Pei, Roy Clarke, Panos Argyrakis, and Raoul Kopelman. 2010. Magnetic confinement of Brownian rotation to a single axis and application to Janus and cluster microparticles. Applied Physics Letters 97, no. 14: 144103. doi:10.1063/1.3485296.
McNaughton, B. H, P. Kinnunen, R. G Smith, S. N Pei, R. Torres-Isea, R. Kopelman, and R. Clarke. “Compact sensor for measuring nonlinear rotational dynamics of driven magnetic microspheres with biomedical applications.” Journal of Magnetism and Magnetic Materials (2009).
Paivo's work highlighted on Mlive.com
University of Michigan
Graduate Program in Immunology (2008-2010)
Project: Understanding the role of adjuvants in the molecular signaling of Th17 cell differentiation
Advisor: James R. Baker Jr., MD
About Shelly: Shelly graduated from the University of Michigan in 2008 with a degree in Biochemistry. She did her undergraduate research in the field of Systems and Synthetic
Biology, focusing on building and characterizing genetic bistable switches to generate memory in E. coli. Her current research interests have shifted towards a more translational focus in the field of Immunology.
Chang DE, Leung S, Reifler AN, Atkinson MR, Forger DB, Ninfa AJ. 2009. Building biological memory by linking positive feedback loops. Proc Natl Acad Sci USA. (Revisions in progress)
Becky Lahti Matz
University of Illinois at Urbana-Champaign
Department of Chemistry (2007-present)
Project: Polyplex Exposure and Protein Expression Inhibit Cell Division
Advisor: Mark Banaszak Holl, PhD
About Becky: Becky graduated with a B.S. in Chemistry from the University of Illinois in 2007 where she did research with Scott Silverman on catalytic DNA. At Michigan, Becky is a fifth-year student working towards a Ph.D. in Chemistry. Her research focuses on understanding mechanisms of non-viral gene delivery and, in particular, cellular response to nanoparticle exposure. She has completed the Rackham Certificate in Nanobiology as well as an M.S. in Post-Secondary Science Education under the guidance of Joe Krajcik at Michigan's School of Education. Becky's CV
3. Khmaladze, A.; Matz, R.L.; Epstein, T.; Jasensky, J.; Zhang, C.; Banaszak Holl, M.M.; Chen, Z. “Cell Volume Changes During Apoptosis Monitored in Real Time Using Digital Holographic Microscopy”, 2011, submitted to Journal of Structural Biology.
2. Matz, R.L.; Rothman, E.D.; Krajcik, J.S.; Banaszak Holl, M.M. “Does Concurrent Enrollment in Lecture and Laboratory Impact Student Performance and Retention?” 2011, submitted to the Journal of Research in Science Teaching.
1. Khmaladze, A.; Matz, R.L.; Zhang, C.; Wang, T.; Banaszak Holl, M.M.; Chen, Z. “Dual-Wavelength Linear Regression Phase Unwrapping in Three-Dimensional Microscopic Images of Cancer Cells”, Opt. Lett. 2011, 36, 912–914. (Selected for publication in the Optical Society of America’s Virtual Journal for Biomedical Optics 2011, 6 (4).)
6. Khmaladze, A.; Matz, R.L.; Jasensky, J.; Epstein, T.; Zhang, C.; Banaszak Holl, M.M.; Chen, Z. “Dynamic Cellular Volume Measurements by Single and Dual-Wavelength Digital Holographic Microscopy”, presented at
the Optical Society of America's Digital Holography and Three Dimensional Imaging Topic Meeting, Tokyo, Japan, 05/2011.
5. Khmaladze, A.; Matz, R.L.; Zhang, C.; Jasensky, J.; Zhang, C.; Banaszak Holl, M.M.; Chen, Z. “Dual Wavelength Digital Holography Phase Unwrapping by Linear Regression”, presented at the SPIE Conference on Defense, Security, and Sensing, Orlando, FL, 04/2011.
4. Matz, R.L. “Research in Graduate School and A Bit of Gene Delivery”, presented at an Undergraduate Research Opportunity Program (UROP) Seminar, University of Michigan, 09/2010.
3. Matz, R.L.; Banaszak Holl, M.M. “Concurrent Versus Nonconcurrent Enrollment in General Chemistry Lecture and Laboratory Courses”, presented at the Biennial Conference on Chemical Education, Laboratory Innovations and Investigations Session, Denton, TX, 08/2010.
2. Matz, R.L.; Rothman, E.D.; Krajcik, J.S.; Banaszak Holl, M.M. “Concurrent Enrollment in General Chemistry Lecture and Laboratory Decreases Withdrawal Rates and Increases Final Grades in the Lecture”, presented at the National Association of Research in Science Teaching Annual Conference, Strand of College Science Teaching and Learning (Grades 13-20), Philadelphia, PA, 03/2010.
1. Matz, R.L. “Establishing if Transfection Requires Degradation of the Nuclear Membrane that Occurs During Mitosis”, presented at the Michigan Nanotechnology Institute for Medicine and Biological Sciences Institute-Wide Meeting, University of Michigan, 03/2009.
7. Matz, R.L.; Banaszak Holl, M.M. “Polyplex Exposure Decreases HeLa Cell Division Rate and Affects Expression of Cell Cycle and Inflammatory Response Genes”, presented at the Department of Chemistry's Vaughan Symposium, University of Michigan, 07/2011.
6. Khmaladze, A.; Matz, R.L.; Jasensky, J.; Epstein, T.; Zhang, C.; Banaszak Holl, M.M.; Chen, Z. “The Study of Apoptotic Morphological Changes by Dual-Wavelength Holographic Microscopy”, presented at the Conference on Lasers and Electro-Optics, Division of Science and Innovations, Baltimore, MD, 05/2011.
5. Matz, R.L.; Banaszak Holl, M.M. “Concurrent Versus Nonconcurrent Enrollment in Physical Science Lecture and Laboratory Courses”, presented at the Center for Research on Learning and Teachings Investigating Student Learning Fair, University of Michigan, 05/2011.
4. Matz, R.L.; Erickson, B.; Li, J.J.; Baker, Jr., J.R.; Orr, B.G.; Banaszak Holl, M.M. “Effect of Polyplex Exposure on Cell Division and Gene Regulation”, presented at the Michigan Nanotechnology Institute for Medicine and Biological Sciences, Herbert D. Doan Symposium on Nanotechnology, University of Michigan, 04/2011.
3. Matz, R.L.; Erickson, B.; Li, J.J.; Orr, B.G.; Baker, Jr., J.R.; Banaszak Holl, M.M. “Polyplex Exposure and Protein Expression Inhibit Cell Division”, presented at the
2. Matz, R.L.; Banaszak Holl, M.M. “Establishing if Transfection Requires Degradation of the Nuclear Membrane that Occurs During Mitosis”, presented at the (a) Department of Chemistry's Vaughan Symposium, University of Michigan, 08/2009; (b) American Chemical Society National Meeting, Division of Biological Chemistry, Washington, D.C., 08/2009; and (c) Michigan Nanotechnology Institute for Medicine and Biological Sciences, Herbert D. Doan Symposium on Nanomaterials and Nanotechniques for Biology, University of Michigan, 09/2009.
1. Kelly, C.V.; Matz, R.L.; Baker, Jr., J.R.; Banaszak Holl, M.M.; Orr, B.G. “How Nanoparticles Interact with Living Cells”, presented at the Michigan Student Assembly's Environmental Issues Commission Sustainability Fair, University of Michigan, 02/2009.
Rahul Rattan, M.S.
Bachelor of Engineering
Panjab University, India, 2006
MSE Biomedical Engineering
University of Michigan, 2008
Project: Impact of dendrimer based drug delivery on mammalian cells
Advisor: Mark Banaszak Holl, PhD
About Rahul: Rahul graduated from Panjab University, India in 2006 with a BE in Biotechnology. He is currently pursuing a PhD in Biomedical Engineering. He is characterizing the impact of dendrimer based drug delivery on mammalian cells, specifically the way these polymers and the polymer/DNA polyplexes interact with cell membranes and enter cells.
Rahul also has a substantial interest in translating ideas to the clinic and has also focused on invention. He was an active participant in the Ross Business School's Dare to Dream program, the 2009 ASME Innovation Showcase (IShow) competition, and was recently a featured inventor at the University of Michigan's annual Celebrate Inventation event. This event showcases inventors from all over campus and most typically highlights faculty led groups. Rahul's invention, the Endocutter, was developed with a group of students originating from a project in a BME course. The Endocutter converts a traditional endoscope into a device that can cut up and remove blood clots, without damaging the stomach, making the identifical of bleeding areas in the stomach much more feasible and efficient. The invention can be viewed at http://www.youtube.com/watch?v=gxnB_kuzOsQ. This is wondeful example of biomedical innovation and points to Rahul's powerful combination of strong science and engineering coupled with entrepreneurship.
2008 Dare to Dream recipient (through University of Michigan Business School).
2008 BME Idea stipend award recipient (through NCIIA)
Ranked 4th in ASME iShow competition 2009 for “Endocutter” device, summer 2009
Twice recipient of grant from Medical Innovation Center for device prototyping, summer and fall 2009
The Role of Ganglioside GM1 in Cellular Internalization Mechanisms of Poly(amidoamine) Dendrimers. S. Hong, R. Rattan, I. Majoros, D. G. Mullen, J. L. Peters, X. Shi, A. U. Bielinska, L. Blanco, B. G. Orr. J. R. Baker, M. M. Banaszak Holl Bioconjugate Chemistry 2009, 20, 1503-1513.
Jiumei Chen, M.S.
Ph.D. program in Macromolecular Science and Engineering
Wayne State University
About Jiumei: She earned a double major at Tianjin University with a Bachelors of Engineering in Polymer Materials and Engineering and a Bachelors of Arts in English.
She then earned an MS in Materials Science and Engineering from Wayne State University. She is now in
the Nanobiology certificate program
using patch clamp techniques to study the interactions between nanoparticles and biological membranes. This work will help to provide an understanding of nanoparticle toxicity and the mechanism of DNA transfection.
October 2006, Materials Incorporated Award in recognition of her academic excellence in the Macromolecular Science and Engineering Center. The award was presented at the banquet of the annual symposium.
2009, the Distinguished Achievement Award from the Macromolecular Science and Engineering Program.
Cationic Nanoparticles Induce Nanoscale Disruption in Living Cell Plasma Membranes
J. Chen, J. A. Hessler, K. Putchakayala, B. K. Panama, D. P. Khan, S. Hong, D. G. Mullen, S. C. DiMaggio, A. Som, G. N. Tew, A. N. Lopatin, J. R. Baker, M. M. Banaszak Holl, B. G. Orr Journal of Physical Chemistry B 2009, 113, 11179-11185.
University of Maryland at College Park
Applied Physics (2007-present)
Pulse-shaping Multi-photon FRET Microscopy
Jennifer Ogilvie, PhD
About Meredith: Meredith graduated cum laude from the University of Maryland at College Park in 2007, where she earned a B.S. with honors in physics, completed a second major in psychology, and was elected to Phi Beta Kappa. Meredith is currently working towards her Ph.D. in Applied Physics, researching the application of laser pulse shaping techniques to nonlinear microscopy. She is a recipient of the NIH Molecular Biophysics Training Grant, the Rackham Merit Fellowship, and the University Regents’ Fellowship. Outside of the lab, Meredith is a member of the University of Michigan Women’s Glee Club.
2nd Place Poster Prize, 2010 M Symposium on Biophysics, University of Michigan Ann Arbor.
“Pulse Shaping Multiphoton FRET Microscopy,” Meredith H. Brenner, Sarah R. Nichols, and Jennifer P. Ogilvie. 2010 Cross-Border Workshop on Laser Science, University of Waterloo.
“Pulse Shaping Multiphoton FRET Microscopy,” Meredith H. Brenner, Sarah R. Nichols, and Jennifer P. Ogilvie, 2010 M Symposium on Biophysics, University of Michigan Ann Arbor.
“Pulse Shaping Multiphoton FRET Microscopy,” Meredith H. Brenner, Sarah R. Nichols, and Jennifer P. Ogilvie. 2009 Herbert D. Doan Nanotechnology Symposium, University of Michigan MNIMBS.
H. Yoon, M. Brenner, J.P. Rice, N. R. Briggs, and G. Gillen, in the Proceedings of SPIE Vol. 6205, Thermosense XXVIII. Edited by Jonathan J. Miles, G. Raymond Peacock, and Kathryn M. Knettel, April 2006.
Ming-Hsin Li, M.S.
PhD student, Biomedical Engineering, University of Michigan, 2007- 2013
BS, Chemical Engineering
Chung Yuan Christian University, 1999
MS, Chemical Engineering
National Taiwan University, 2001
Advisors: James R. Baker, MD and Seok Ki Choi, PhD
Project 1: Dendrimer-based multivalent methotrexates as bi-functional therapeutics
Folate binding protein (FBP)-targeted nanodevice has been widely studied to treat site-specifically on tumor cells and tissues for decades, already showing therapeutic efficacy in vitro and in vivo. Based on this rational design, we covalently conjugated methotrexate (MTX), a weakly binding ligand of FBP, onto poly(amidoamine) generation 5 (PAMAM G5) dendrimer to construct a potential targeting polymer therapeutic. To demonstrate binding affinity of the MTX-nanoconjugate to FBP and multivalency-enhanced avidity, a surface plasmon resonance (SPR) biosensor was applied to measure for this system. According to results of SPR, the multivalent MTX-coupled PAMAM dendrimer showed enhanced avidity to immobilized FBP. We also conducted cell based experiments to test the biological activity of this nanodevice. The MTX-presented PAMAM dendrimer was able to cause cytotoxicty in FBP-overexpressed cells.
Project 2: engineering synthetic mimics of DNA-protein recognition systems
Recognition of protein on specific DNA segment initiates subsequent gene expressions and launches diversely enormous biological reactions. This accurate recognition process is believed that a preceding non-specific searching, mainly electrostatic interaction, facilitates the followed specific targeting. In this study we intend to engineer surface charge on PAMAM dendrimer, to mimic DNA-binding protein and simulate its behavior on DNA segments. By using single molecule detection technique, we are able to visualize the process in real-time.
Awards and Honors
President's Award, Chung Yuan Christian University (1999)
The 2nd Prize of Poster Context, 39th Annual of Chinese Society of Microbiology (2005)
Study abroad Fellowship, Ministry of Education, Taiwan (2007~2008)
US Patent: Novel Biological Flocculants and Production Methods (Published Application No. 20040219619651)
Ming-Hsin Li, Shang-Ting Tsai, Chung-Hsuan Chen, Chiu Wen Chen, Yuan Tseh Lee, Yi-Sheng Wang, Sensitive Bipolar Ion Detector Based on Sequential Conversion Reactions, Analytical Chemistry 2007, 79, 1277-1282 (cover article)
Choi, S K, Thomas, T, Li, M-H, Kotlyar, A, Desai, A, Baker, J R, Jr. Light-controlled release of caged doxorubicin from folate receptor-targeting PAMAM dendrimer nanoconjugate, Chem. Commun. 2010, 46, 2632-2634
Thomas, T, Choi, S K, Li, M-H, Kotlyar, A, Baker, J R, Jr. Design of riboflavin-presenting PAMAM dendrimers as a new nanoplatform for cancer-targeted delivery. Bioorg. & Med. Chem. Lett. 2010, 17, 5191-5194
Plantinga A, Witte A, Li MH, Harmon A, Choi SK, Banaszak Holl MM, Orr BG, Baker JR Jr, Sinniah K. Bioanalytical Screening of Riboflavin Antagonists for Targeted Drug Delivery - A Thermodynamic and Kinetic Study. ACS Med Chem Lett. 2011 May 12;2(5):363-367. PMCID: PMC3114641
Choi SK, Leroueil P, Li MH, Desai A, Zong H., Van Der Spek AFL, Baker JR Jr. Specificity and Negative Cooperativity in Dendrimer-Oxime Drug Complexation. Macromolecules (Communication), 2011;44:4026-4029.
Li M-H, Choi SK, Thomas TP, Desai A, Lee K-H, Kotlyar A, Banaszak Holl MM, Baker JR Jr. Dendrimer-based Multivalent Methotrexates as Dual Acting Nanoconjugates for Cancer Cell Targeting. Eur. J. Med. Chem. 2012; 47: 560-572. PMCID: PMC3243070
Yu S, Li MH, Choi SK, Baker JR, Larson RG. DNA Condesation by Partially Acetylated Poly(amido amine) Dendrimers: Effects of Dendrimer Charge Density on Complex Formation. Molecules. 2013 Sep 3;18(9):10707-20. PMID 24005965
Cline EN, Li M-H, Choi SK, Herbstman JF, Kaul N, Meyhöfer E, Skiniotis G, Baker Jr. JR, Larson RG, Walter NG. Paclitaxel-Conjugated PAMAM Dendrimers Adversely Affect Microtubule Structure through Two Independent Modes of Action. Biomacromolecules 2013, 14, 654-664. PCMID: PMC3603340
Ming-Hsin Li, Seok Ki Choi, Thommey P. Thomas, Alina Kotlyar, Suyang Qin, James R baker, Dendrimer-based multivalent methotrexates as dual acting nanoconjugates in cancer cell targeting, 4th annual NCI alliance for nanotechnology in cancer investigators meeting, Manhattan Beach, CA, Oct 20-22, 2009.
Asish Misra, B.S.
MD/PhD Program, University of Michigan
SB.S. Chemical Engineering, MIT, 2008
SB.S. Electrical Engineering and Computer Science, MIT 2008
Minors in Math, Biomedical Engineering
About Asish Misra: During his research rotation in MNIMBS, Asish Misra' reseach was focused on macrophages that have been implicated in many disease processes, from synovial inflammation in rheumatoid arthritis to angiogenesis in tumors. The macrophages in these pathophysiologies often overexpress particular receptors that can be exploited to deliver therapeutics exclusively to these cells. In particular, we are studying the folate receptor and mannose receptors as potential targets.
C. J. Bettinger, J. P. Bruggeman, A. Misra, J. T. Borenstein, and R. Langer. Biocompatibility of biodegradable semiconducting melanin films for nerve tissue engineering. Biomaterials, vol. 30, no. 17, pp. 3050-3057, 2009.
C.J. Bettinger, B. Orrick, A. Misra, R. Langer and J.T. Borenstein. Microfabrication of poly(glycerol-sebacate) for contact guidance applications. Biomaterials 27, pp. 2558–2565, 2006.
T. R. F. Fulford-Jones, D. Ruan, W. R. Chan, K. Hartman, A. M. Heafitz, A. C. Misra, T. B. Mloduchowski. The Mars Gravity Biosatellite: Innovations in Murine Motion Analysis and Life Support. Proceedings of the 2005 International Conference on Environmental Systems, July 2005.
Btech Indian Institute of Technology, Kharagpur
PhD precandidate, Mechanical Engineering
Project: microthermal modulator for 2dimensional gas chromatography; Biomolecular Motor Smart Microarrays: Self-Contained, High-Throughput, Ultrasensitive Multiplexed Biomolecular Sensing
About Dibyadeep: Dibyadeep graduated from IIT Kharagpur, with honours, and completed a masters degree in mechanical Engineering at Iowa State Unviersity, with specialization in MEMS, microfluidics and microfabrication.
- "Microchannel Flow Control through a Combined Magneto-Electro-Hydrodynamic Transport",D Paul and S Chakraborty,
Journal of Physics. D, Applied Physics, 39, 5364 (2006).
- "Wall effects in Microchannel based Macromolecular Separation under Electro-Magneto-Hydrodynamic Influences” D Paul and S Chakraborty,
Journal of Applied Physics,102, 074921 (2007)
Virtual Journal of Biological Physics Research, 15th October’2007
- "Modeling and Simulation of a novel pressure sensor, based on thermal transpiration" D Paul and S Mahalingam,
J. Phys.: Conf. Ser. 114 012062 (2008)
- “Effect of Streaming current on Transport of cylindrical Particles through a microchannel” D Paul, and S Chakraborty (submitted, Langmuir)
- “Single Cell trapping using spiral Microfluidic device” P Ramesh, D Paul and L Dong, (under construction)
- “Nafion mediated electro-dissolution of Copper substrate under DC voltage” D Paul, A Bastawros, A Chandra, H Johnson , ASME Global Congress on NanoEngineering for Medicine and Biology’2010
- “Focused electric field induced ion transport” A Bastawros, A Chandra, KH Law, D Paul, H Johnson, A Semichaevsky, NSF Engineering Research and Innovation Conference, Honululu, Hawaii, June 22-25’2009
- "Ion transport in focused electric field lithography" D Paul, A Bastawros, A Chandra, H Johnson, 121st Annual Meeting of the Iowa Academy of Science, Friday and Saturday, April 17-18’2009